This research was purposed to obtain the information about the evidence of Tuberculosis (TB) infectious disease on long tail macaque (Macaca fascicularis) in tourism area Weh Island, Sabang and to support the government's program in generating Indonesia free TB, as well. This research was conducted on many stages, those were permits arrangement with BKSDA Aceh and Forestry Officer Sabang, location identification of long tail macaque populations, and identification of the amount of long tail macaque population in each groups. The monkeys chosen were restrained and anaesthetized using Ketamin (10mg/kg) and xylazine (2mg/kg) intramuscularly. Screening test using tuberculin skin test (TST) method (old mammalian tuberculin kit) was carried out to all samples, 0.1 mL (135000 Tuberculin Unit) on palpebrae intradermally. The effect of TST was examined at 24, 48, and 72 hours post treatment. The test was repeated on the second week. The data were tabulated and analyzed descriptively. From the identification of macaca populations in two areas was found one group of macaca in Kilometer Nol (N 05° 54' 19,1" E 95° 12' 57,4") with the population of 14 macacas whereas in Cot Murong area (N 05° 54' 19,1" E 95° 12' 57,4") found two groups with the population of 12 and 14 macacas. TB screening test using TST method showed that there were no incidents of TB in long tail macaque in Sabang tourism area.