To improve sexual health among people living with HIV, sexual health should be addressed duringconsultations in routine HIV care. The aim of the present study was to investigate to what extentSexual Health Counselling (SHC) is incorporated into routine Dutch HIV care and to exploredifferences between physicians and nurses in their practices and views regarding SHC. A cross-sectional survey was conducted among all HIV physicians (N=110) and HIV nurses (N=82) in theNetherlands. A questionnaire assessed socio-demographic characteristics, current SHC practice,topics addressed, and factors associated with engaging in SHC. The response rate was 53.6%(N=59) among physicians and 60.0% (N=40) among nurses. SHC was performed by 26.1% ofphysicians and 83.9% of nurses (Χ² (1) = 27.68,p<.001). The most frequently reported barrier forSHC was the presence of a third party, endorsed by 50.9% of physicians and 60.4% of nurses.Nurses were more likely to address issues related to sexual wellbeing, while physicians mainlydiscussed medical topics. While, both HIV physicians and nursed felt responsible for providingSHC, nurses were more likely to address SHC that physicians. There is scope for improving SHCfor PLHIV through a multidisciplinary approach based on clear guidelines for physicians and nurses.