Quality and quantity of DNA isolated from frozen urine in population-based research
In several population-based studies in the past urine samples were collected and stored for future research. We set out to determine the reliability of using such samples for genotyping DNA markers in epidemiologic research. A source of DNA extracted from exfoliated nucleated cells in urine is provided by the DOM cohort, in which specimens were collected 15-25 years ago. We have examined the quality of the DNA in 48 of these samples by measuring the amount of DNA isolated and its ability to provide an adequate PCR template for amplicons of different lengths. MTHFR polymorphism was analyzed in... Mehr ...
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Dokumenttyp: | Artikel |
Erscheinungsdatum: | 2002 |
Schlagwörter: | DNA / DNA Primers / Epidemiologic Methods / Female / Filtration / Freezing / Genotype / Humans / Methylenetetrahydrofolate Reductase (NADPH2) / Netherlands / Observer Variation / Oxidoreductases Acting on CH-NH Group Donors / Polymerase Chain Reaction / Polymorphism / Genetic / Specimen Handling / Journal Article / Research Support / Non-U.S. Gov't |
Sprache: | Englisch |
Permalink: | https://search.fid-benelux.de/Record/base-26834151 |
Datenquelle: | BASE; Originalkatalog |
Powered By: | BASE |
Link(s) : | https://dspace.library.uu.nl/handle/1874/11791 |