Intratracheal administration of solutions in mice; development and validation of an optimized method with improved efficacy, reproducibility and accuracy
Animal models are still vital in the field of respiratory disease research. To improve the accuracy and consistency of the dose of specific compounds administered specifically in the respiratory tract, it is important to optimize and to compare the technique to currently available techniques. In this study, an optimized intubation-mediated intratracheal administration (IMIT) technique is described and compared to oropharyngeal aspiration (OA). Adult female Balb/c mice were treated with Evans Blue using IMIT or OA and sacrificed after a short recovery to observe the distribution of solutions th... Mehr ...
Verfasser: |
Pelgrim, Charlotte E
van Ark, Ingrid
Leusink-Muis, Thea
Brans, Maike A D
Braber, Saskia
Garssen, Johan
van Helvoort, Ardy
Kraneveld, Aletta D
Folkerts, Gert |
Dokumenttyp: |
Artikel |
Erscheinungsdatum: |
2022 |
Reihe/Periodikum: |
Pelgrim , C E , van Ark , I , Leusink-Muis , T , Brans , M A D , Braber , S , Garssen , J , van Helvoort , A , Kraneveld , A D & Folkerts , G 2022 , ' Intratracheal administration of solutions in mice; development and validation of an optimized method with improved efficacy, reproducibility and accuracy ' , Journal of Pharmacological and Toxicological Methods , vol. 114 , 107156 . https://doi.org/10.1016/j.vascn.2022.107156 |
Schlagwörter: |
Animals
/ Bronchoalveolar Lavage Fluid
/ Disease Models
/ Animal
/ Female
/ Intubation
/ Intratracheal
/ Lipopolysaccharides
/ Lung
/ Mice
/ Inbred BALB C
/ Reproducibility of Results
/ Research Design
/ Oropharyngeal
/ APOPTOSIS
/ INSTILLATION
/ MOUSE
/ Netherlands
/ LIPOPOLYSACCHARIDE
/ Methods
/ EXPOSURE |
Sprache: |
Englisch |
Permalink: |
https://search.fid-benelux.de/Record/base-26821875 |
Datenquelle: |
BASE;
Originalkatalog |
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BASE |
Link(s) :
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https://cris.maastrichtuniversity.nl/en/publications/a50346e4-41c5-4e63-a436-4a59b18f6b14 |