O1-S03.03 Clonally related Neisseria gonorrhoeae isolates with decreased susceptibility to extended-spectrum cephalosporins in Amsterdam, the Netherlands

Background Between 2006 and 2008, the prevalence of Neisseria gonorrhoeae (NG) isolates with decreased susceptibility (0.125 0.125&emsp14;μg/ml (group A), 54 with a CTX MIC of 0.125&emsp14;μg/ml (group B), and a control group of 74 with a CTX MIC of <0.125&emsp14;μg/ml (group C), were included. All isolates were characterised using antibiograms, conventional penA mosaic gene PCR, and genotyping by NG-multi-locus variable-number tandem repeat analysis (MLVA). PenA mosaic positive isolates and a strict selection of the remaining isolates were further characterised by NG-multilocus... Mehr ...