K-ras oncogene mutations in sporadic colorectal cancer in The Netherlands Cohort Study

Activation of K-ras oncogene has been implicated in colorectal carcinogenesis, being mutated in 30–60% of the adenocarcinomas. In this study, 737 incident colorectal cancer (CRC) patients, originating from 120 852 men and women (55–69 years at baseline) participating in the Netherlands Cohort Study (NLCS), were studied in order to evaluate subgroups with respect to K-ras mutation status. Mutation analysis of the exon 1 fragment of the K-ras oncogene, spanning codons 8–29, was performed on archival colorectal adenocarcinoma samples of all patients using macrodissection, nested PCR and direct se... Mehr ...