Diagnosis and epidemiology of Trichinella infections in wildlife in the Netherlands

Trichinella infections in foxes and wild boars were studied to determine the prevalence of infection in wildlife in the Netherlands. Muscles of 429 forelegs of foxes (Vulpes vulpes) and diaphragms of 11 wild boars (Sus scrofa) were artificially digested. Single larvae of Trichinella were identified at species level using random amplified polymorphic DNA (RAPD-PCR). In addition, an ELISA using ES antigen was used to test serum samples of 458 wild boars. The prevalence of Trichinella In foxes ranged from 3.9 % in the the eastern part of the country, 13.1 % in the central part of the country and... Mehr ...