Development and Validation of a S1 Protein-Based ELISA for the Specific Detection of Antibodies against Equine Coronavirus
Equine coronavirus (ECoV) is considered to be involved in enteric diseases in foals. Recently, several outbreaks of ECoV infection have also been reported in adult horses from the USA, France and Japan. Epidemiological studies of ECoV infection are still limited, and the seroprevalence of ECoV infection in Europe is unknown. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) method utilizing ECoV spike S1 protein was developed in two formats, and further validated by analyzing 27 paired serum samples (acute and convalescent sera) from horses involved in an ECoV outbreak and 1... Mehr ...
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Dokumenttyp: | Artikel |
Erscheinungsdatum: | 2019 |
Reihe/Periodikum: | Viruses, vol 11, iss 12 |
Verlag/Hrsg.: |
eScholarship
University of California |
Schlagwörter: | Microbiology / Biological Sciences / Prevention / Emerging Infectious Diseases / Vaccine Related / Biodefense / Infection / Animals / Antibodies / Neutralizing / Viral / Betacoronavirus 1 / Coronavirus Infections / Enzyme-Linked Immunosorbent Assay / Horse Diseases / Horses / Iceland / Netherlands / ROC Curve / Seroepidemiologic Studies / Serologic Tests / Spike Glycoprotein / Coronavirus / equine coronavirus / spike S1 protein / ELISA / virus neutralization / seroprevalence |
Sprache: | unknown |
Permalink: | https://search.fid-benelux.de/Record/base-26791930 |
Datenquelle: | BASE; Originalkatalog |
Powered By: | BASE |
Link(s) : | https://escholarship.org/uc/item/9v5572vz |