International audience ; and are roundworms of dogs, cats and pigs, respectively, that can also infect humans. These zoonotic helminths have a worldwide distribution and are also endemic in the Netherlands. Infection with may result in visceral larva migrans (VLM) or ocular larva migrans (OLM) caused by the migrating larvae. Although has been reported to occasionally mature to the adult stage in humans, clinical cases of VLM suspected to be caused by have been described. Diagnosis of these helminth infections relies mainly on serology. Here we analyse the results from the and IgG-ELISA from a total of 2,838 serum samples from VLM and OLM suspected patients that were sent to our institution from 1998 to 2009. Results indicate that for each year the seropositivity is significantly higher compared to the seropositivity. Furthermore, while seropositivity has decreased over time, the seropositivity has not significantly changed for the past 12 years. The and seropositivity was also shown to increases with age and, while gender has no influence on the seropositivity, males showed higher seropositivity.