© 2018, Serbian Genetics Society. The comparative estimation was carried out with seven tomato hybrids (Lycopersicon esculentum Mill.) during the period 2011 to 2013 in greenhouse at Agricultural Institute of Republic of Srpska, Banja Luka, Bosnia and Herzegovina. The aim of this study is to examine the dry matter content, lycopene in fruits and total yield of Dutch tomato hybrids in the conditions of western Bosnia and Herzegovina. The experiment was set up by a randomized block design in three repetitions. The dry matter content ranged from 5.60% to 8.14%. Hybrid and hybrid x year interaction had significant (p < 0.05) influence on lycopene content and tomato yield. The factors that had a decisive influence on the content of dry matter, lycopene content in fruit and tomato yield were year, hybrid and cultivation technology.