Prior to this study, the moth flies in The Netherlands were represented by 61 species. Our findings derive from a citizen-science expedition in the Vondelpark in Amsterdam, one of the oldest public parks and best known parks in The Netherlands. The combination of citizen science and the exploration of a well-known urban park has allowed us to contribute to the knowledge of moth fly species present in The Netherlands. The findings from this study provide valuable insights into the distribution, taxonomy and genetic resources of Psychoda and Panimerus species, enhancing our understanding of insect biodiversity and promoting future research in this field. Our study provides two new geographical records of the moth flies in The Netherlands, namely, Psychoda uniformata Haseman, 1907 and Panimerus maynei (Tonnoir, 1920) elevating the total number of species to 63. Furthermore, we provide re-descriptions of the females of Panimerus notabilis (Eaton, 1893) and P. goetghebueri (Tonnoir, 1919). Additionally, we make available for the first time, the sequence of the 5’-end of the cytochrome c oxidase subunit I ( COI ) gene or COI Barcodes for Panimerus notabilis , P. goetghebueri and P. maynei . These COI Barcodes serve as valuable tools for future species identification within the genus.