Micropropagation of Ulmus minor and U. minor x U. pumila from 4-year-old ramets ; Micropropagación de Ulmus minor y U. minor x U. pumila a partir de ramets de 4 años de edad

As part of the Spanish breeding programme against Dutch elm disease, investigations were undertaken on reproduction of Ulmus minor by means of tissue culture. Microshoots were obtained from nodal segments, collected from terminal twigs of 4-year-old ramets of selected clones of this species and of U. minor x U. pumila, and cultured on a modified Murashige & Skoog medium containing 6-Benzylaminopurine (BA). When subcultured, the microshoots or nodal segments excised from these, formed new shoots, their maximum numbers per explant (1.94 to 2) being obtained with the hybrid clone M-TC2 on med... Mehr ...