Maize is one of the most commonly grown crops in Belgium and is primarily cultivated as animal feed. At sowing, maize seeds are coated with synthetic fungicides and/or repellents in order to protect them from damping-off or from birds. The aim of this study was to identify one biological control agent (BCA) which is naturally able to control soil-borne fungal agents causing damping-off. More than 1,000 native fungi were isolated from Belgian maize agroecosystems. In vitro screenings were carried out to determine their antagonistic activities against Fusarium avenaceum and F. culmorum fungi at 25°C, 15°C, 10°C and 5°C and their abilities to produce lytic enzymes. This led to the identification of the forty best BCA candidates. The BC0584 strain with the highest antagonistic activity was identified as Trichoderma atroviride. Its ability to control soil-borne fungal damping-off pathogens was then tested in planta. Bioassays conducted in greenhouses revealed that inoculating the substrate with the T. atroviride endophytic strain significantly improves the emergence rate of seedlings infected by F. avenaceum or F. culmorum, from 20.0 ± 10.0 % to 85.0 ± 11.2 % and from 60.0 ± 7.1 % to 95.0 ± 8.7 %, respectively (P<0.001). Two field trials were then performed during the season 2017 and the coating of maize seeds with T. atroviride conidia significantly increased the emergence rate of naturally-infected seedlings by 28.2 ± 5.8 % and by 23.6 ± 9.6 % compared to untreated seeds. Modes of action and required conditions to ensure biocontrol in fields have still to be investigated. In order to develop an efficient and viable formulation of the BCA, and to consider its concrete development from production, and storage to its application on the field, three seed coating formulations were compared under controlled conditions. One slurriable powder allows to maintain conidia viability to 84.1% ± 14.3 % over a six month-storage period at 4°C in the dark. This formulation allows for spores to be easily, quickly and cheaply ...