Development of a Radioimmunoassay for Bovine Chymosin B

The present study was conducted to develop and validate a specific radioimmunoassay system for measurement of bovine chymosin B (bChyB) concentrations in plasma samples. Bovine ChyB was used for immunization of rabbits and as standard and tracer. Chymosin B concentrations were measured in plasma samples from two groups of calves (Group 1: calves sampled from birth to 24 hours; Group 2: calves sampled from Day 1 to 21 after birth) and from one cow during the peri-partum period. Detection limit of the assay was 9.0 ng/ml. Recovery was higher than 89.3%. Repeatability and reproducibility ranged f... Mehr ...