The Dangers of Using Cq to Quantify Nucleic Acid in Biological Samples: A Lesson From COVID-19
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA quantities, measured by reverse transcription quantitative PCR (RT-qPCR), have been proposed to stratify clinical risk or determine analytical performance targets. We investigated reproducibility and how setting diagnostic cutoffs altered the clinical sensitivity of coronavirus disease 2019 (COVID-19) testing. Methods: Quantitative SARS-CoV-2 RNA distributions [quantification cycle (Cq) and copies/mL] from more than 6000 patients from 3 clinical laboratories in United Kingdom, Belgium, and the Republic of Korea were a... Mehr ...
Dokumenttyp: | Artikel |
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Erscheinungsdatum: | 2022 |
Verlag/Hrsg.: |
American Association For Clinical Chemistry
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Schlagwörter: | Belgium / COVID-19 Nucleic Acid Testing / standards* / COVID-19* / diagnosis / Humans / Nucleic Acids* / analysis / RNA / Viral / analysis / Reproducibility of Results / Republic of Korea / SARS-CoV-2 / Sensitivity and Specificity / United Kingdom / RT-qPCR / cycle threshold / quantification cycle |
Sprache: | Englisch |
Permalink: | https://search.fid-benelux.de/Record/base-26580324 |
Datenquelle: | BASE; Originalkatalog |
Powered By: | BASE |
Link(s) : | https://ir.ymlib.yonsei.ac.kr/handle/22282913/188118 |