Retrospective evaluation of routine whole genome sequencing of Mycobacterium tuberculosis at the Belgian National Reference Center, 2019
Objectives: Since January 2019, the Belgian National Reference Center for Mycobacteria (NRC) has switched from conventional typing to prospective whole-genome sequencing (WGS) of all submitted Mycobacterium tuberculosis complex (MTB) isolates. The ISO17025 validated procedure starts with semi-automated extraction and purification of gDNA directly from the submitted MGIT tubes, without preceding subculturing. All samples are then sequenced on an Illumina MiSeq sequencer and analyzed using an in-house developed and validated bioinformatics workflow to determine the species and antimicrobial resi... Mehr ...
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Dokumenttyp: | Dataset |
Erscheinungsdatum: | 2021 |
Schlagwörter: | Genetics / Pharmacology / Infectious Diseases / Environmental Sciences not elsewhere classified / Biological Sciences not elsewhere classified / Whole-genome sequencing / tuberculosis / resistance / diagnostic / bioinformatic |
Sprache: | unknown |
Permalink: | https://search.fid-benelux.de/Record/base-26525500 |
Datenquelle: | BASE; Originalkatalog |
Powered By: | BASE |
Link(s) : | https://doi.org/10.6084/m9.figshare.16964875.v1 |