Detection and characterization of class A extended-spectrum-{beta}-lactamase-producing Pseudomonas aeruginosa isolates in Belgian hospitals

Objectives To investigate the presence of extended-spectrum β-lactamases (ESBLs) among Pseudomonas aeruginosa clinical isolates referred to two Belgian reference laboratories. Methods Antibiograms were analysed for P. aeruginosa isolates referred between 2004 and 2008. Isolates resistant to ceftazidime (MIC > 8 mg/L) and with a positive double-disc synergy test between ceftazidime and clavulanate were serotyped and screened for the presence of ESBL-encoding genes. Genes encoding metallo-β-lactamases ( bla MBL ) were sought by PCR in ESBL-producing isolates with positive imipenem/EDTA synerg... Mehr ...