SACCHARIFICATION ENZYMATIC APPLIED TO THE FRESH PEA POD VARIETY PISUM SATIVUM L. FOR OBTAIN REDUCING SUGAR. ; Sacarificación enzimática de la vaina de arveja fresca (pisum sativum l.) variedad sabanera para obtención de azúcares reductores

This study describes the process of enzymatic saccharification applied to the fresh pea pod variety Santa Isabel, a content rich vegetable material lignocellulose, which can be exploited to obtain sugars. For effects of the research they thought about to apply nine treatments keeping in mind: the solvent type (deionized water and sulfuric acid), the enzyme type (Novozymes® and Alkozym®), the so much dilution relationship of the solvent like of the enzyme, one also kept in mind the use or not of autoclave like part of the treatment applied to the samples. The qualitative and quantitative determ... Mehr ...

Verfasser: González González, Simón Andrés
Dokumenttyp: Artikel
Erscheinungsdatum: 2019
Verlag/Hrsg.: Universidad Nacional Abierta y a Distancia
UNAD
Schlagwörter: enzyme / hydrolysis / hydrolyzed / lignocellulosic material / saccharides / enzima / hidrólisis / hidrolizado / material lignocelulósico / sacáridos / SIN INFORMACION
Sprache: Spanish
Permalink: https://search.fid-benelux.de/Record/base-28834736
Datenquelle: BASE; Originalkatalog
Powered By: BASE
Link(s) : http://hemeroteca.unad.edu.co/index.php/publicaciones-e-investigacion/article/view/579

This study describes the process of enzymatic saccharification applied to the fresh pea pod variety Santa Isabel, a content rich vegetable material lignocellulose, which can be exploited to obtain sugars. For effects of the research they thought about to apply nine treatments keeping in mind: the solvent type (deionized water and sulfuric acid), the enzyme type (Novozymes® and Alkozym®), the so much dilution relationship of the solvent like of the enzyme, one also kept in mind the use or not of autoclave like part of the treatment applied to the samples. The qualitative and quantitative determination of the sugars was carried out for liquid chromatography of high resolution (HPLC) using a team LC-2010 AHT Shimadzu®, with a detector of refraction index RID-10A Shimadzu® and a column Shodex SC1011, using deionized water as the phase mobile. Similarly it was a chemical characterization of the pea pods certain percentage of moisture, protein, fiber, fat and ash. It also performed analysis of Lignin, holocellulose, cellulose and hemicellulose. The Statistical analysis for the content of sugars was performed by analysis of variance and comparison of means by Tukey test, in order to find the best treatment; Finding that treatment 8, the works best for obtaining glucose and fructose, but considering not to use sulfuric acid, the best treatment would be 4; which applies to all three sugars identified and quantified (sucrose, glucose and fructose). For data obtained from the analysis of lignin, holocellulose, cellulose and hemicellulose was applied to analysis of variance to establish whether the samples subjected to hydrolysis treatments show a significant difference in content of lignocellulosic material ; En este estudio se describe el proceso de sacarificación enzimática aplicada a la vaina de arveja fresca, variedad Santa Isabel, material vegetal rico en contenido lignocelulósico que puede ser aprovechado para la obtención de azúcares. Para efectos de la investigación se planteó aplicar nueve tratamientos, teniendo ...