Abstract Background Regular physical activity has multiple benefits for physical, mental, and psychosocial, however many students do not meet the national physical activity guidelines. Classroom physical activity can help students be more physically active. This study investigated the attitude of university students towards classroom physical activity breaks in a university of applied science setting Methods Within this research a mixed methods design have been applied. A online survey was completed by 2016 students an semi-structured interviews were conducted among 19 students. Results A total of 2016 students successfully completed the questionnaire with a mean of 18-21 year (67%) and 52 % female. No classroom physical activity breaks were used during the lessons. In addition, almost half of the students are not able to concentrate well during the classes. Students (39%) noticed that physical activity during classes had a positive influence on their concentration. The majority of the students are open to activating forms of teaching at universities of applied sciences (69%). Students have a clear preference for active breaks, curriculum-focused active breaks. Physical activities such as climbing stairs, sports exercises are not students preferred activities. Conclusions Implementing classroom physical activity breaks can improve student physical activity and concentration during school and behavior in the classroom. Activities such as climbing stairs, intense aerobic exercise were perceived as inconvenient. Active breaks, curriculum-focused active breaks were acknowledged by students. Key messages Keep physical activity breaks short and manageable. Physical activity breaks can help in stress reduction and improving psychological well-being.