Establecimiento de condiciones in vitro para la inducción de Morfogénesis en Dioon merolae De Luca, Sabato y Vázquez Torres (Zamiaceae) especie en peligro de extinción

Organogenic cultures were induced from zygotic embryo and megagametophyte explants of the Chiapas State (México) endangered cycad species, Dioon merolae De Luca Sabato & Vazquez Torres. The induction medium consisted of B5 major salts, Murashige and Skoog (1962) minor salts and organics, 400 mg/L glutamine, 100 mg/L arginine, 100 mg/L asparagine, 60 g/L, sucrose, 4 g gellan gum, and supplemented with 2,4- Dichlorophenoxyacetic acid (2,4-D) (0, 0.45, 2.26, 4.52, 9.05 ųM) and kinetin (0, 2.32, 4.60, 9.30, 13.90 ųM) arranged as a 5 x 5 factorial in a randomized block design. Cultures were mai... Mehr ...