A Belgian Serosurveillance/Seroprevalence Study of Diphtheria, Tetanus and Pertussis Using a Luminex xMAP Technology-Based Pentaplex
Serosurveillance and seroprevalence studies are an essential tool to monitor vaccine-preventable diseases. We have developed a magnetic bead-based pentaplex immunoassay (MIA) for the simultaneous detection of IgG antibodies against diphtheria toxin (DT), tetanus toxin (TT), pertussis toxin (PT), filamentous hemagglutinin (FHA) and pertactin (Prn). The in-house pentaplex MIA showed a good correlation with commercial ELISAs with correlation coefficients between 0.89 for PT and 0.98 for TT. Intra- and inter-assay variability was <10%. A total of 670 anonymized serum samples collected in 2012 i... Mehr ...
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Dokumenttyp: | Text |
Erscheinungsdatum: | 2016 |
Verlag/Hrsg.: |
Multidisciplinary Digital Publishing Institute
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Schlagwörter: | seroprevalence / serosurveillance / pertussis / diphtheria / tetanus / multiplex |
Sprache: | Englisch |
Permalink: | https://search.fid-benelux.de/Record/base-26512709 |
Datenquelle: | BASE; Originalkatalog |
Powered By: | BASE |
Link(s) : | https://doi.org/10.3390/vaccines4020016 |